Production of extracellular lipase by Enterococcus faecium E68 with olive oil waste as substrate

With green technologies, the damages caused by agro-technological wastes to environment are eliminated. In our study, it was aimed both prevent harm of olive oil waste and produce lipase enzyme, which is an important biotechnological product.. E. faecium E68 obtained from milk dairy products used in production enzyme. developed medium containing 10% waste, pH6.5, at 37oC with 120 rpm agitation for 48 hours. The effect temperature, pH metal ion, surfactant NaCl also determined.
 molecular weight partially purified extracellular enzyme estimated be around 19-20 kDa SDS-PAGE. optimum temperature 45°C, while exhibited appreciable thermostability retaining activity 55°C 48h. most active range 3-11 10. 1mM, Ca 2+, Mn2+, Cu2+, Ni2+, Zn2+, Mg2+ K+ ions modulated but inhibited Hg2+, SDS Triton X-100. halophilic 25% salt increased activity.
 Olive may preferred substrate production.